Plasmid

Part:BBa_K5005014:Design

Designed by: Yufan Liu   Group: iGEM23_GEC-CHINA   (2023-10-10)


pTarget


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 294
    Illegal NotI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1987
    Illegal BglII site found at 2053
    Illegal BglII site found at 2094
    Illegal BglII site found at 5518
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 839
    Illegal NgoMIV site found at 1872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2076
    Illegal BsaI site found at 5500
    Illegal BsaI.rc site found at 792
    Illegal BsaI.rc site found at 3941
    Illegal SapI site found at 2858
    Illegal SapI.rc site found at 6149


Design Notes

Target plasmid contains a T7 promoter downstream of the CMV promoter. The target gene will be inserted into the multiple cloning site downstream of the T7 promoter. This T7 promoter enables the editor to perform gene editing specifically on the target gene inserted after the T7 promoter.


Source

Constructed from the literature.

References

Chen, H., Liu, S., Padula, S., Lesman, D., Griswold, K., Lin, A., Zhao, T., Marshall, J. L., & Chen, F. (2020). Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. Nature biotechnology, 38(2), 165–168. https://doi.org/10.1038/s41587-019-0331-8